Development and validation of a high-throughput analysis of glutathione in grapes, musts and wines by Stable Isotope Dilution Assay and LC–MS/MS. (15th June 2015)

• Record Type: Journal Article
• Authors: Roland, Aurélie; Schneider, Rémi
• Is Part Of: Food chemistry. Volume 177(2015)
• Page Start: 152
• Page End: 157
• Publication Date: 2015-06-15
• Subjects: SIDA Stable Isotope Dilution Assay -- MRM Multiple Reaction Monitoring -- HPLC-Chip/MS high performance liquid chromatography hyphenated with a chip cube and a mass spectrometer -- ESI electrospray ionization -- RSD residual standard deviation -- GSH glutathione -- ISTD internal standard; Glutathione -- Stable Isotope Dilution Assay -- Grapes -- Wines -- Validation; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2015.01.027
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: We proposed a high-throughput method to analyze the reduced glutathione. We need only 1 mL of sample to perform the analysis. We have a very accurate method using SIDA quantification. Abstract: For the first time, we proposed a high-throughput method to quantify glutathione in grapes, musts and wines for all grape varieties using Stable Isotope Dilution Assay (SIDA). Indeed, the use of SIDA as a quantification method is essential to overcome the chemical instability of glutathione. In practice, glutathione was derivatized in-situ with N-ethylmaleimide to block the cysteine residue and to enhance its lipophilic properties. After quenching with acetic acid, samples were directly analyzed by LC–MS/MS (run of 13 min) in Multiple Reaction Monitoring mode using labeled glutathione as internal standard. The validation according to the International Organization of Vine and Wine recommendations demonstrated the high sensitivity (LOD = 45 μg L −1 ), accuracy (recovery = 112%) and intermediate reproducibility (RSD = 12%) of the method. This high-throughput method that requires only 1 mL of matrix, allowed us to analyze 70 samples per day for a moderate cost.