Differential protein profiles of postharvest Gynura bicolor D.C leaf treated by 1-methylcyclopropene and ethephon. (1st June 2015)

• Record Type: Journal Article
• Authors: Jiang, Li; Kang, Ruoyi; Zhang, Li; Jiang, Juan; Yu, Zhifang
• Is Part Of: Food chemistry. Volume 176(2015)
• Page Start: 27
• Page End: 39
• Publication Date: 2015-06-01
• Subjects: G. bicolor -- Senescence -- 1-Methylcyclopropene -- Ethephon -- Proteomics; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2014.11.081
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: This is the first proteomic study involving postharvest leaf vegetables. A proteomic approach was used to study the effect of 1-MCP on G. bicolor . 1-MCP decelerated the metabolism of G. bicolor leaf and enhanced its defense. 1-MCP promoted gene transcription, translation and protein expression. 1-MCP and ethylene exerted opposite effects on maintain the integrity of cells. Abstract: Proteins were extracted from G. bicolor that had been treated with 1-methylcyclopropene and ethephon and then stored at room temperature for 1, 3 and 7 days. More than 300 protein spots were detected by 2-DE and 38 differentially abundant spots ( P < 0.05) were excised and analysed by using MALDI-TOF/TOF. Thirty-three proteins were finally confidently identified. According to the Clusters of Orthologous Groups of proteins, the proteins identified were classified into those responsible for metabolism (75.8%), information storage and processing (9.1%) and cellular processes and signaling (12.1%). Compared with ethephon and control treatments, 1-methylcyclopropene specifically increased the abundances of superoxide dismutase, peroxidase, carbonic anhydrase, nucleoside diphosphate kinases, glyceraldehyde 3-phosphate dehydrogenase, RuBisCO and ribulose bisphosphate carboxylase/oxygenase activase. 1-Methylcyclopropene protected leaf chloroplast and cells by enhancing stress response and defense, and delayed senescence by inhibiting substance and energy metabolisms. Therefore, 1-methylcyclopropene allowed better self-defense and delayed senescence of G. bicolor leaf.