Comparative study of the bioconversion process using R-(+)- and S-(–)-limonene as substrates for Fusarium oxysporum 152B. (1st May 2015)

• Record Type: Journal Article
• Authors: Molina, Gustavo; Bution, Murillo L.; Bicas, Juliano L.; Dolder, Mary Anne Heidi; Pastore, Gláucia M.
• Is Part Of: Food chemistry. Volume 174(2015)
• Page Start: 606
• Page End: 613
• Publication Date: 2015-05-01
• Subjects: Monoterpenes -- Limonene-1, 2-diol -- α-Terpineol -- Anaerobic biotransformation -- Cell permeabilisation; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2014.11.059
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Complete process comparison of R -(+)- and S -(–)-limonene fungal bioconversion. Characterisation of the bioconversion of S -(–)-limonene into limonene-1, 2-diol. One of the highest concentrations obtained of α-terpineol and limonene-1, 2-diol. First report to characterise the bioconversion using ultra-structural analysis. Abstract: This study compared the bioconversion process of S -(–)-limonene into limonene-1, 2-diol with the already established biotransformation of R -(+)-limonene into α-terpineol using the same biocatalyst in both processes, Fusarium oxysporum 152B. The bioconversion of the S -(−)-isomer was tested on cell permeabilisation under anaerobic conditions and using a biphasic system. When submitted to permeabilisation trials, this biocatalyst has shown a relatively high resistance; still, no production of limonene-1, 2-diol and a loss of activity of the biocatalyst were observed after intense cell treatment, indicating a complete loss of cell viability. Furthermore, the results showed that this process can be characterised as an aerobic system that was catalysed by limonene-1, 2-epoxide hydrolase, had an intracellular nature and was cofactor-dependent because the final product was not detected by an anaerobic process. Finally, this is the first report to characterise the bioconversion of R -(+)- and S -(–)-limonene by cellular detoxification using ultra-structural analysis.