Effects of alkaline pretreatments and acid extraction conditions on the acid-soluble collagen from grass carp (Ctenopharyngodon idella) skin. (1st April 2015)

• Record Type: Journal Article
• Authors: Liu, Dasong; Wei, Guanmian; Li, Tiancheng; Hu, Jinhua; Lu, Naiyan; Regenstein, Joe M.; Zhou, Peng
• Is Part Of: Food chemistry. Volume 172(2015)
• Page Start: 836
• Page End: 843
• Publication Date: 2015-04-01
• Subjects: Grass carp -- Skin -- Acid-soluble collagen -- Alkaline pretreatment -- Acid extraction -- Temperature; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2014.09.147
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: 0.05–0.1 M NaOH removed non-collagenous protein without ASC loss and modification. 0.2–0.5 M NaOH removed ASC, and 0.5 M NaOH modified ASC structure at 15–20 °C. 0.5–1.0 M acetic acid almost fully extracted ASC without structural modification. Recommended condition to produce ASC: 0.05–0.1 M NaOH, 0.5 M acetic acid, 4–20 °C. Processing temperature can be adjusted with season instead of commonly used 4 °C. Abstract: This study investigated the effects of alkaline pretreatments and acid extraction conditions on the production of acid-soluble collagen (ASC) from grass carp skin. For alkaline pretreatment, 0.05 and 0.1 M NaOH removed non-collagenous proteins without significant loss of ASC at 4, 10, 15 and 20 °C; while 0.2 and 0.5 M NaOH caused significant loss of ASC, and 0.5 M NaOH caused structural modification of ASC at 15 and 20 °C. For acid extraction at 4, 10, 15 and 20 °C, ASC was partly extracted by 0.1 and 0.2 M acetic acid, while 0.5 and 1.0 M acetic acid resulted in almost complete extraction. The processing conditions involving 0.05–0.1 M NaOH for pretreatment, 0.5 M acetic acid for extraction and 4–20 °C for both pretreatment and extraction, produced ASC with the structural integrity being well maintained and hence were recommended to prepare ASC from grass carp skin in practical application.