Fat-soluble vitamin intestinal absorption: Absorption sites in the intestine and interactions for absorption. (1st April 2015)

• Record Type: Journal Article
• Authors: Goncalves, Aurélie; Roi, Stéphanie; Nowicki, Marion; Dhaussy, Amélie; Huertas, Alain; Amiot, Marie-Josèphe; Reboul, Emmanuelle
• Is Part Of: Food chemistry. Volume 172(2015)
• Page Start: 155
• Page End: 160
• Publication Date: 2015-04-01
• Subjects: FSVs fat-soluble vitamins -- FBS fetal bovine serum -- wt mice wild-type mice -- PDA photodiode array detector -- SR-BI Scavenger Receptor class B type I -- CD36 Cluster Determinant 36 -- NPC1-L1 Niemann–Pick C1-Like 1; Retinol -- Cholecalciferol -- Tocopherol -- Phylloquinone -- Digestion -- Enterocyte; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2014.09.021
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Vitamin A, D, E and K display different absorption sites in mouse intestine. Vitamin E may prevent vitamin A oxidation and increases its intestinal absorption. Vitamin D, E and K common uptake pathways would explain the competitions observed. Abstract: The interactions occurring at the intestinal level between the fat-soluble vitamins A, D, E and K (FSVs) are poorly documented. We first determined each FSV absorption profile along the duodenal–colonic axis of mouse intestine to clarify their respective absorption sites. We then investigated the interactions between FSVs during their uptake by Caco-2 cells. Our data show that vitamin A was mostly absorbed in the mouse proximal intestine, while vitamin D was absorbed in the median intestine, and vitamin E and K in the distal intestine. Significant competitive interactions for uptake were then elucidated among vitamin D, E and K, supporting the hypothesis of common absorption pathways. Vitamin A also significantly decreased the uptake of the other FSVs but, conversely, its uptake was not impaired by vitamins D and K and even promoted by vitamin E. These results should be taken into account, especially for supplement formulation, to optimise FSV absorption.