Development of a monoclonal antibody-based indirect competitive immunosorbent assay for 4(5)-Methylimidazole detection in caramels. (1st March 2015)

• Record Type: Journal Article
• Authors: Wu, Xin-Lan; Yu, Shu-Juan; Kang, Ke-Ren
• Is Part Of: Food chemistry. Volume 170(2015)
• Page Start: 354
• Page End: 359
• Publication Date: 2015-03-01
• Subjects: 4(5)-Methylimidazole -- Monoclonal antibodies -- ic-ELISA; 4(5)-Methylimidazole (PubChem CID: 13195) -- 1-Methylimidazole (PubChem CID: 1390) -- Histamine (PubChem CID: 774) -- 2-Methylimidazole (PubChem CID: 12749) -- 1, 2-Dimethylimidazole (PubChem CID: 5617) -- 5-Benzimidazolecarboxylic acid (PubChem CID: 459456) -- 4-Nitrobenzyl bromide (PubChem CID: 66011); Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2014.07.148
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Hapten of 4-MI has been successfully prepared. Ic-ELISA method to test 4-MI based on monoclonal antibody has been established. The established ic-ELISA method was sensitive and specific for 4-MI detection in caramels. Abstract: In this study, an indirect competitive enzyme-linked immunoassay (ic-ELISA) based on monoclonal antibody for 4(5)-Methylimidazole (4-MI) detection was described. The artificial antigens were prepared by conjugating bovine serum albumin (BSA) or ovalbumin (OVA) with the hapten of 4-MI. And monoclonal antibody, evaluated by ic-ELISA, was obtained by immunizing BABL/c mice. After optimizing, a standard curve for ic-ELISA detection on 4-MI was obtained with the linear detection range of 0.64–20.48 mg/L. The cross-reactivity (CR) of all the structural analogues of 4-MI was less than 5.62%. The recoveries of 4-MI in caramels detection were ranged from 88.69% to 114.09%, with relative standard deviation ( n = 3) below 8.07%. The results suggested that the established ic-ELISA is promising for 4-MI commercial detection in caramels.