Antioxidant activity and protective effect of extract of Celosia cristata L. flower on tert-butyl hydroperoxide-induced oxidative hepatotoxicity. (1st February 2015)

• Record Type: Journal Article
• Authors: Kim, Yon-Suk; Hwang, Jin-Woo; Sung, Si-Heung; Jeon, You-Jin; Jeong, Jae-Hyun; Jeon, Byong-Tae; Moon, Sang-Ho; Park, Pyo-Jam
• Is Part Of: Food chemistry. Volume 168(2015)
• Page Start: 572
• Page End: 579
• Publication Date: 2015-02-01
• Subjects: Celosia cristata L -- Antioxidant activity -- Free radical -- t-BHP -- Chang cells -- Oxidative stress; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2014.07.106
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Study of antioxidant and protective effect of Celosia cristata L. flower extracts. Oxidative damage was induced in Chang cells and rat livers by t -BHP. CCF enhanced cell viability and mitochondrial membrane depolarisation. Oral feeding of CCF in rats lowered serum levels of GOT and GPT. CCF decreased hepatic levels of LPO and serum TG in t -BHP-induced hepatotoxicity. Abstract: This study was undertaken to evaluate the antioxidant potential and protective effects of Celosia cristata L. (Family: Amaranthaceae) flower (CCF) extracts on tert -butyl-hydroperoxide ( t -BHP)-induced oxidative damage in the hepatocytes of Chang cells and rat livers. In vitro, CCF extracts exhibited protective effect through their radical scavenging ability to enhance cell viability, prevent reactive oxygen species (ROS) generation, and inhibit mitochondrial membrane depolarisation in t -BHP-induced hepatotoxicity in Chang cells. In vivo, oral feeding of CCF (100 mg and 500 mg/kg of body weight) to rats for five consecutive days before a single dose of t -BHP (2 mmol/kg, i.p.) showed a significant ( p < 0.05) protective effect by lowering serum levels of glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT). The extract decreased the hepatic levels of lipid peroxidation (MDA) and serum level of triglyceride (TG) against t -BHP-induced oxidative stress. These results indicate that CCF extract prevented oxidative stress-induced liver injury by enhancing hepatocyte antioxidant abilities.