Authentication of pineapple (Ananas comosus [L.] Merr.) fruit maturity stages by quantitative analysis of γ- and δ-lactones using headspace solid-phase microextraction and chirospecific gas chromatography–selected ion monitoring mass spectrometry (HS-SPME–GC–SIM-MS). (1st February 2015)

• Record Type: Journal Article
• Authors: Steingass, Christof B.; Langen, Johannes; Carle, Reinhold; Schmarr, Hans-Georg
• Is Part Of: Food chemistry. Volume 168(2015)
• Page Start: 496
• Page End: 503
• Publication Date: 2015-02-01
• Subjects: Post-harvest handling -- Fruit logistics -- Lactone enantiomers -- Authentication; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2014.07.071
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Chirospecific analysis of lactones in pineapples of different ripening stages. HS-SPME–GC–SIM-MS and stable isotope dilution assay (SIDA) based quantification. Air-freighted fruits characterised by γ-hexalactone (γ-C6) of high enantiomeric purity. Increased ( S )-γ-C6 genesis during post-harvest ripening of sea-freighted pineapples. Authentication of pineapple fruits according to their post-harvest procedures. Abstract: Headspace solid phase microextraction and chirospecific gas chromatography–mass spectrometry in selected ion monitoring mode (HS-SPME–GC–SIM-MS) allowed quantitative determination of δ-lactones (δ-C8, δ-C10) and γ-lactones (γ-C6, γ-C8, γ-C10). A stable isotope dilution assay (SIDA) with d7 -γ-decalactone as internal standard was used for quantitative analysis of pineapple lactones that was performed at three progressing post-harvest stages of fully ripe air-freighted and green-ripe sea-freighted fruits, covering the relevant shelf-life of the fruits. Fresh pineapples harvested at full maturity were characterised by γ-C6 of high enantiomeric purity remaining stable during the whole post-harvest period. In contrast, the enantiomeric purity of γ-C6 significantly decreased during post-harvest storage of sea-freighted pineapples. The biogenetical background and the potential of chirospecific analysis of lactones for authentication and quality evaluation of fresh pineapple fruits are discussed.