Agent orange herbicides, organophosphate and triazinic pesticides analysis in olive oil and industrial oil mill waste effluents using new organic phase immunosensors. (15th February 2015)

• Record Type: Journal Article
• Authors: Martini, Elisabetta; Merola, Giovanni; Tomassetti, Mauro; Campanella, Luigi
• Is Part Of: Food chemistry. Volume 169(2015)
• Page Start: 358
• Page End: 365
• Publication Date: 2015-02-15
• Subjects: Immunosensors -- Organic phase -- Triazinic -- Organophosphate and chlorurate pesticides -- Analysis -- Olive oil -- Mill waste effluents; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2014.07.137
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: New immunosensors working in organic solvent mixtures (OPIEs) for analysis of pesticides. The linear response of about 10 nM and 5.0 μM was obtained in presence of olive oil. Recovery tests were carried out in commercial or artisanal extra virgin olive oil. Traces of pesticides were checked in oily matrix, in pomace oil and mill wastewaters. Immunosensors show good selectivity and satisfactorily precision. Abstract: New immunosensors working in organic solvent mixtures (OPIEs) for the analysis of traces of different pesticides (triazinic, organophosphates and chlorurates) present in hydrophobic matrices such as olive oil were developed and tested. A Clark electrode was used as transducer and peroxidase enzyme as marker. The competitive process took place in a chloroform–hexane 50% (V/V) mixture, while the subsequent enzymatic final measurement was performed in decane and using tert-butylhydroperoxide as substrate of the enzymatic reaction. A linear response of between about 10 nM and 5.0 μM was usually obtained in the presence of olive oil. Recovery tests were carried out in commercial or artisanal extra virgin olive oil. Traces of pesticides were also checked in the oily matrix, in pomace and mill wastewaters from an industrial oil mill. Immunosensors show good selectivity and satisfactory precision and recovery tests performed in olive oil gave excellent results.