Two-step biocatalytic reaction using recombinant Escherichia coli cells for efficient production of phenyllactic acid from l-phenylalanine. (January 2018)

• Record Type: Journal Article
• Authors: Zhao, Weirui; Ding, Huanru; Lv, Changjiang; Hu, Sheng; Huang, Jun; Zheng, Xiaodong; Yao, Shanjing; Mei, Lehe
• Is Part Of: Process biochemistry. Volume 64(2018)
• Page Start: 31
• Page End: 37
• Publication Date: 2018-01
• Subjects: 3-phenyllactic acid -- l-phenylalanine -- Membrane-bound l-amino acid deaminases -- d-2-hydroxyisocaproate dehydrogenase -- Formate dehydrogenase; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2017.09.019
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: A two-step bioprocess for efficient production of phenyllactic acid was developed. 1st: phenylalanine was converted to phenylpyruvic acid by E. coli expressing mL-AAD. 2nd: phenylpyruvic acid converted to phenyllactic acid by a coupling system. 121 mM phenyllactic acid was produced from 150 mM phenylalanine with a yield of 81%. This process was promising for industrial production of phenyllactic acid. Abstract: 3-Phenyllactic acid (PLA) is a natural product with broad and effective antimicrobial activity that is often used as a preservative in the food industry and as feed additive to replace antibiotics in livestock feeds. In this study, a two-step biocatalytic process for production of PLA from l-phenylalanine was developed and investigated. In the first step, l-phenylalanine was converted to phenylpyruvic acid (PPA) by recombinant Escherichia coli cells expressing membrane-bound l-amino acid deaminase from Proteus vulgaris . In the second step, unpurified PPA was directly converted to PLA by recombinant E. coli cells co-expressing d-2-hydroxyisocaproate dehydrogenase from Lactobacillus delbrueckii subsp. bulgaricus and formate dehydrogenase from Mycobacterium vaccae N10. Through two-step cascade reactions, 121 mM PLA was produced from 150 mM l-phenylalanine with a yield of 81.3%. Thus, this process showed great production efficiency and is a promising method for industrial production of PLA.