Co-digestion of Rhodosporidium toruloides biorefinery wastes for biogas production. (January 2018)

• Record Type: Journal Article
• Authors: Marques, Isabel Paula; Batista, Ana Paula; Coelho, Aldo; Lopes da Silva, Teresa
• Is Part Of: Process biochemistry. Volume 64(2018)
• Page Start: 221
• Page End: 227
• Publication Date: 2018-01
• Subjects: R. toruloides -- Yeast biorefinery -- Wastes mixtures -- Co-digestion -- Biogas; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2017.09.023
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: R. toruloides biomass biorefinery wastes were used for biogas production. Various wastes mixtures were anaerobically co-digested for biogas production. The best mixture contained biomass residue and supernatant with glucose. Flow cytometry evaluated the effect of the mixtures on the bacterial consortium. The co-digestion mitigated the negative effect of the toxics present in the wastes. Abstract: Rhodosporiodum toruloides NCYC 921 yeast biorefinery is able to simultaneously provide a gaseous energy carrier, lipids and high-value carotenoids, either using glucose or carob pulp syrup as carbon source in the cultivation step for biomass production. The resulting leftover materials from the yeast biorefinery (YR- de-oiled yeast biomass residue, GS- glucose supernatant, CS- carob supernatant and CR- carob residue) were valued through anaerobic digestion in different mixtures, according to the generated effluents volume. Biogas productions of 55, 31 and 29 mL and methane yield of 236, 179 and 144 mL CH4 /g substrate volatile solids (VS) were recorded for the studied mixtures MIX I (YR + GS), MIX II (YR + CS) and MIX III (YR + CS + CR) respectively. The comparison between the biogas production and volatile solids removal obtained for the individual substrates and for the admixtures substrates digestions revealed that co-digestion favoured the conversion of the biorefinery wastes into biogas/methane. Flow cytometry analysis of cells stained with propidium iodide revealed that the proportion of cells with injured membrane was lower in the substrate mixtures digestions than in the individual waste digestions, indicating that co-digestion mitigates the negative effect of potential toxic compounds present in the wastes or produced during the process digestion on the microbial consortium.