Integrated transcriptomic and metabolomic analysis of Rhizopus oryzae with different morphologies. (January 2018)

• Record Type: Journal Article
• Authors: Xu, Qing; Fu, Yongqian; Li, Shuang; Jiang, Ling; Rongfeng, Guan; Huang, He
• Is Part Of: Process biochemistry. Volume 64(2018)
• Page Start: 74
• Page End: 82
• Publication Date: 2018-01
• Subjects: Rhizopus oryzae -- Transcriptomics -- Metabolomics -- Morphology; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2017.10.001
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Integrated omics were performed with different morphologies samples. Serious oxidative stress was presented in pellet sample. To adapt stress, cells changed the wall component to form pellet. Uniform pellet was formed in a broad pH area by increasing the chitosan contained. Abstract: In trying to discover the potential genes or strategies used to regulate the morphology of Rhizopus oryzae, cells in different morphological states were subjected to transcriptomic and metabolomic analyses. The results showed that the tricarboxylic acid cycle was down-regulated in the pellet sample, whereas the metabolism of pyruvic acid, unsaturated fatty acids, and amino acids, as well as the synthesis of sugar alcohols, were up-regulated. Moreover, an increase of oxidative stress markers was detected during pellet formation. In response to this stress, R. oryzae up-regulated the aforementioned pathways and induced a number of antioxidant enzymes. In addition, R. oryzae adapted its cell wall composition to reduce the surface area exposed to the environment. Following these findings, hemicellulose hydrolysate was added into the culture medium, which led to the formation of uniform pellets over a broad pH range. This study might provide clues to deduce the unequivocal general relationships between process variables and fungal morphology.