Lipase production and purification by self-buffering ionic liquid-based aqueous biphasic systems. (December 2017)

• Record Type: Journal Article
• Authors: Lee, Sze Ying; Khoiroh, Ianatul; Coutinho, João A.P.; Show, Pau Loke; Ventura, Sónia P.M.
• Is Part Of: Process biochemistry. Volume 63(2017)
• Page Start: 221
• Page End: 228
• Publication Date: 2017-12
• Subjects: Lipase from Burkholderia cepacia ST8 -- Good's buffer ionic liquids -- Integrated downstream process -- Aqueous biphasic systems; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2017.08.020
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Aqueous biphasic systems composed of self-buffering ionic liquids were used for the purification of an extracellular lipase from its fermentation broth. Highlights: Self-buffering aqueous biphasic systems formed with GB-ILs, salts, and (co)polymers. Lipase preferential partition towards the GB-IL-rich phase. An extracellular lipase produced via submerged fermentation was studied. An efficient downstream process to recover a microbial lipase was developed. Abstract: In this work, a group of Good's buffer ionic liquids (GB-ILs) comprised of tetrabutylammonium, tetrabutylphosphonium and cholinium cations paired with Good's buffer (GB) anions (MOPSO, BES and TAPSO) was studied. Their distinctive capability to induce the formation of aqueous biphasic systems (ABS) with the salts K3 PO4, K2 CO3, and (NH4 )2 SO4, and the polymers poly(ethylene glycol) (PEG), poly(propylene glycol) (PPG), and PEG-PPG copolymers was demonstrated. Their application as purification tools to recover a lipase produced via submerged fermentation by Burkholderia cepacia ST8 was investigated. The lipase was preferentially partitioned towards the GB-IL-rich phase in both the GB-IL + salt and polymer + GB-IL purification systems. Molecular docking studies were performed aiming at to understand the possible interactions between the GB-IL ions and the lipase residues. Furthermore, the selected GB-IL-based ABS was investigated as part of an integrated process developed to successfully recover and purify an extracellular B. cepacia ST8 lipase from the fermentation broth, in which a purification factor of 22.4 ± 0.7 and a recovery yield of (94.0 ± 0.2) % were achieved.