Prevalence, characterization and antimicrobial susceptibility of Yersinia enterocolitica and other Yersinia species found in fruits and vegetables from the European Union. (March 2018)

• Record Type: Journal Article
• Authors: Verbikova, Veronika; Borilova, Gabriela; Babak, Vladimir; Moravkova, Monika
• Is Part Of: Food control. Volume 85(2018)
• Page Start: 161
• Page End: 167
• Publication Date: 2018-03
• Subjects: Vegetable -- Strawberry -- Yersinia enterocolitica -- Zoonosis -- Antibiotic resistance; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2017.08.038
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: A total of 312 samples of fruits, vegetables and sprouts originating from the European Union states were examined for the presence of pathogenic and non-pathogenic Yersinia enterocolitica and other Yersinia spp. The samples were examined separately using both cultivation method and real-time quantitative PCR (qPCR) without pre-enrichment. The prevalence of Yersinia spp. in examined samples assessed by cultivation was 17.0% (53/312), with Y. enterocolitica and other Yersinia spp. comprising 5.8% (18/312) and 11.2% (35/312) of samples, respectively. The ail gene, which is associated with Y. enterocolitica pathogenicity, was detected only in one isolate (0.3%). Biotyping of isolates revealed only biotype 1A. Serotyping tests showed that four isolates belonged to serotype O:8 and one to serotype O:5. QPCR results confirmed the presence of Yersinia spp. and pathogenic Y. enterocolitica (carrying the ail gene) in 37 (11.9%) and 21 (6.7%) samples, respectively. Further, the susceptibility of Y. enterocolitica and the other Yersinia spp. isolates was tested using the agar dilution method and the Vitek ® 2 system. All samples were susceptible to ciprofloxacin, gentamicin, enrofloxacin, cefotaxime, imipenem, meropenem, tobramycin, ciprofloxacin, colistin, trimethoprim and trimethoprim/sulfamethoxazole. The most commonly found resistance was to ampicillin (100%), followed by erythromycin (98.1%), amoxicillin/clavulanic acid (75.5%) and streptomycin (39.6%). Highlights: Prevalence of Yersinia enterocolitica and other Yersinia spp. in food of plant origin was determined. The samples were examined using cultivation methods and by direct real-time PCR. Biotyping of isolates revealed only biotype 1A. High level of antibiotic resistance in Y. enterocolitica and other Yersinia spp. was observed.