Arcobacter spp. at retail food from Portugal: Prevalence, genotyping and antibiotics resistance. (March 2018)

• Record Type: Journal Article
• Authors: Vicente-Martins, Sofia; Oleastro, Mónica; Domingues, Fernanda C.; Ferreira, Susana
• Is Part Of: Food control. Volume 85(2018)
• Page Start: 107
• Page End: 112
• Publication Date: 2018-03
• Subjects: Retail food products -- Ready-to-eat food products -- Arcobacter spp. -- Cross-contamination -- Antimicrobial resistance; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2017.09.024
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: Arcobacter genus is considered a foodborne emergent pathogen able to cause disease among humans and animals. The wide distribution and high prevalence of Arcobacter in food is becoming a concern, since consumption of contaminated food and water is considered the most probable route of Arcobacter transmission to humans. In this work, ready-to-eat packaged vegetables, poultry, pork, beef and fish meat were purchased from several retail markets and supermarkets and tested for the presence of Arcobacter spp. Suspected colonies were isolated and identified, characterized through ERIC-PCR and resistance to nine antibiotics commonly used to treat infections by this microorganism was assessed; in parallel, Arcobacter spp. was directly detected on enrichment broth by multiplex PCR. The results show a high prevalence of Arcobacter spp. among retail food, with an overall prevalence of 60.5% obtained by both molecular and culture detection. A. butzleri was the most frequently isolated species (58.5%) followed by A. cryaerophilus (35.8%) and A. skirrowii (5.7%). A high genetic diversity among the isolates was observed, despite the detection of a possible cross-contamination between food samples. Also, a high rate of multidrug resistance among the isolates (85.7%) was observed. Taken together, our results suggest that the consumption of Arcobacter -contaminated food products is of public health concern. Highlights: Arcobacter spp. was found in 60.5% of the retail foods collected in Portugal. A. butzleri was the most frequently isolated species followed by A. cryaerophilus and A. skirrowii. A possible cross-contamination between different food samples was founded. 85.7% of the analysed isolates presented multidrug resistance to antibiotics.