The potential of atmospheric air cold plasma for control of bacterial contaminants relevant to cereal grain production. (December 2017)

• Record Type: Journal Article
• Authors: Los, Agata; Ziuzina, Dana; Boehm, Daniela; Cullen, Patrick J.; Bourke, Paula
• Is Part Of: Innovative food science & emerging technologies. Volume 44(2017)
• Page Start: 36
• Page End: 45
• Publication Date: 2017-12
• Subjects: Cold plasma -- Bacterial biofilms -- B. atrophaeus endospores -- Contact angle -- SEM; Food -- Biotechnology -- Periodicals; Food industry and trade -- Technological innovations -- Periodicals; Aliments -- Biotechnologie -- Périodiques; Food -- Biotechnology; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.ifset.2017.08.008
• Languages: English
• ISSNs: 1466-8564

Abstract

Abstract: The aim of this work was to investigate the efficacy of dielectric barrier discharge atmospheric cold plasma (DBD ACP) against bacteria associated with grains quality and safety. ACP inactivation efficacy was tested against biofilms formed by different strains of E. coli, Bacillus and Lactobacillus in grain model media and against B. atrophaeus endospores either in grain media or attached on abiotic surfaces. Effects were dependent on bacterial strain, media composition and mode of ACP exposure. ACP treatment for 5 min reduced E. coli spp., B. subtilis and Lactobacillus spp. biofilms by > 3 log10, whereas insignificant reductions were achieved for B. atrophaeus . ACP treatment of 5–20 min reduced B. atrophaeus spores in liquids by > 5 log10 . Treatment for 30 min reduced spores on hydrophobic surface by > 6 log10, whereas maximum of 4.4 log reductions were achieved with spores attached to hydrophilic surface. Microscopy demonstrated that ACP caused significant damage to spores. In package ACP treatment has potential to inactivate grain contaminants in the form of biofilms, as well as spores and vegetative cells. Industrial relevance: This study demonstrates that ACP technology is a promising tool for effective bio-decontamination which offers a wide range of possible applications including inactivation of microorganisms on cereal grains. However, due to the nature of the microbial contamination of grains and complex grain structures it may be necessary to optimise the potential for surface inactivation at several stages of grain processing and storage to enhance ACP efficacy against bacterial endospores. Highlights: ACP decreased microbial contamination in cereal-based media and on abiotic surfaces. Process parameters were critical to achieve successful decontamination. Bacterial mode and physiological state affected efficacy of ACP. ACP technology is a promising tool for cereal grain decontamination.