Enzymatic synthesis of rare sugars with l-rhamnulose-1-phosphate aldolase from Thermotoga maritima MSB8. Issue 18 (15th September 2015)

• Record Type: Journal Article
• Authors: Li, Zijie; Wu, Xiaoru; Cai, Li; Duan, Shenglin; Liu, Jia; Yuan, Peng; Nakanishi, Hideki; Gao, Xiao-Dong
• Is Part Of: Bioorganic & medicinal chemistry letters. Volume 25:Issue 18(2015)
• Page Start: 3980
• Page End: 3983
• Publication Date: 2015-09-15
• Subjects: Aldolase -- Rare sugar -- Synthesis -- RhaD -- Thermotoga maritima; Bioorganic chemistry -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1016/j.bmcl.2015.07.027
• Languages: English
• ISSNs: 0960-894X

Abstract

Graphical abstract: Abstract: l -Rhamnulose-1-phosphate aldolase from a thermophilic source ( Thermotoga maritima MSB8) (RhaD T.mari ) was heterologously overexpressed in Escherichia coli and the stereoselectivity of this enzyme with or without Nus tag was investigated. We also applied this enzyme to the synthesis of rare sugarsd -psicose, d -sorbose, l -tagatose andl -fructose using our one-pot four-enzyme system. To the best of our knowledge, this is the first use of RhaD from a thermophilic source for rare sugar synthesis and the temperature tolerance of this enzyme paves the path for large scale fermentation.