Enzymatic synthesis of rare sugars with l-rhamnulose-1-phosphate aldolase from Thermotoga maritima MSB8. Issue 18 (15th September 2015) - Li, Zijie
Graphical abstract: Abstract: l -Rhamnulose-1-phosphate aldolase from a thermophilic source ( Thermotoga maritima MSB8) (RhaD T.mari ) was heterologously overexpressed in Escherichia coli and the stereoselectivity of this enzyme with or without Nus tag was investigated. We also applied this enzyme to the synthesis of rare sugarsd -psicose, d -sorbose, l -tagatose andl -fructose using our one-pot four-enzyme system. To the best of our knowledge, this is the first use of RhaD from a thermophilic source for rare sugar synthesis and the temperature tolerance of this enzyme paves the path for large scale fermentation.