Genome-wide association mapping of starch granule size distribution in common wheat. (September 2017)

• Record Type: Journal Article
• Authors: Li, Jieyun; Rasheed, Awais; Guo, Qi; Dong, Yan; Liu, Jindong; Xia, Xianchun; Zhang, Yan; He, Zhonghu
• Is Part Of: Journal of cereal science. Volume 77(2017)
• Page Start: 211
• Page End: 218
• Publication Date: 2017-09
• Subjects: 90K SNP assay -- GWAS -- SG -- Triticum aestivum; GWAS genome-wide association study -- MAF minor allele frequency -- MAS marker-assisted selection -- MTA marker-trait association -- PIC polymorphism information content -- QTL quantitative trait loci -- SG starch granule -- SGP starch granule bound protein -- SNP single nucleotide polymorphism; Grain -- Periodicals; Cereal products -- Periodicals; Céréales -- Périodiques; Produits céréaliers -- Périodiques; Cereal products; Grain; Periodicals; 664.705
• DOI: 10.1016/j.jcs.2017.08.016
• Languages: English
• ISSNs: 0733-5210

Abstract

Abstract: Starch is a crucial component in wheat endosperm and plays an important role in processing quality. Endosperm of matured wheat grains contains two distinct starch granules (SG), referred to as larger A- and smaller B-granules. In the present study, 166 Chinese bread wheat cultivars planted in four environments were characterized for variation in SG size. A genome-wide association study (GWAS) using the 90 K SNP assay identified 23 loci for percentage volumes of A- and B-granules, and 25 loci for the ratio of A-/B-granules volumes, distributing on 15 chromosomes. Fifteen MTAs were associated with both the percentage volumes of A-, B-granules and the ratio of A-/B-granules volumes. MTAs IWB34623 and IWA3693 on chromosome 7A and IWB22624 and IWA4574 on chromosome 7B associated with the percentage volumes of A- and B-granules consistently identified in multiple environments were considered to be stable. Linear regression analysis showed a significantly negative correlation of the number of favorable alleles with the percentage volumes of A-granules and a significantly positive correlation between the number of favorable alleles and the percentage volumes of B-granules, respectively. The loci identified in this study and associated markers could provide basis for manipulating SG size to obtain superior noodle quality in wheat. Highlights: A GWAS identified 23 loci for percentage volumes of A- and B-granules, and 25 loci for the ratio of A-/B-granules volumes. Fifteen MTAs were associated with both the percentage volumes of A-, B-granules and the ratio of A-/B-granules volumes. MTAs IWB34623, IWA3693, IWB22624 and IWA4574 associated with the percentage volumes of A- and B-granules were stable. The loci identified in this study and associated markers is helpful to obtain superior noodle quality in wheat.