Blue mussel (Mytilus edulis) protein hydrolysate promotes mouse mesenchymal stem cell differentiation into osteoblasts through up-regulation of bone morphogenetic protein. (1st March 2018)

• Record Type: Journal Article
• Authors: Hyung, Jun-Ho; Ahn, Chang-Bum; Je, Jae-Young
• Is Part Of: Food chemistry. Volume 242(2018)
• Page Start: 156
• Page End: 161
• Publication Date: 2018-03-01
• Subjects: Blue mussel -- Enzymatic hydrolysis -- Osteoblast -- Bone morphogenetic protein -- Noggin; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2017.09.043
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Blue mussel protein hydrolysates (BMPH) were produced by enzymatic hydrolysis. BMPH < 1 kDa promoted MSCs differentiation into osteoblast. Activations of BMP-2 signal pathways by BMPH < 1 kDa were involved in osteoblast differentiation. MAPK pathway was also involved in osteoblast differentiation. Abstract: Seafood provides a range of health benefits due to its high-protein level. In this study, the osteogenic effect of blue mussel ( Mytilus edulis ) protein hydrolysates (BMPH) on osteoblast differentiation were examined using mouse mesenchymal stem cells (MSCs). A preparation we called BMPH < 1 kDa which showed the highest osteogenic effect in MSCs, was prepared by peptic hydrolysis. BMPH < 1 kDa treatment stimulated osteoblast differentiation with alkaline phosphatase (ALP) induction, osteocalcin and type I collagen activity as well as calcium deposition. Osteoblast differentiation stimulated by BMPH < 1 kDa treatment was achieved by expression of osteogenic lineage markers, such as bone morphogenetic protein-2 (BMP-2), and downstream signal and transcription factors, including p-Smad1/5/8, Dlx5, runt-related transcription factor 2 (Runx2), and osterix. BMPH < 1 kDa activated phosphorylation of mitogen-activated protein kinases. Adding noggin, a BMP antagonist, inhibited BMPH < 1 kDa-induced ALP activity in MSCs. Taken together, our results show that BMPH < 1 kDa promoted osteoblast differentiation by activating BMP-2.