Determining Neu5Ac in infant formula with ultra‐performance liquid chromatography–tandem mass spectrometry. (3rd February 2015)

• Record Type: Journal Article
• Authors: Li, Chun; Liu, Libo; Xie, Honglei; Liu, Ning
• Is Part Of: International journal of dairy technology. Volume 68:Number 2(2015:May)
• Page Start: 166
• Page End: 173
• Publication Date: 2015-02-03
• Subjects: Infant formulas -- Sialic acid -- Ultra‐performance liquid chromatography–tandem mass spectrometry (UPLC‐MS/MS) -- Determination; Dairying -- Periodicals; Dairying -- Great Britain -- Periodicals; 637
• DOI: 10.1111/1471-0307.12204
• Languages: English
• ISSNs: 1364-727X

Abstract

Abstract : The aim of this work was to measure N‐acetylneuraminic acid (Neu5Ac) in milk‐based infant formulas. The analysis was performed by ultra‐performance liquid chromatography–tandem mass spectrometry (UPLC‐MS/MS). The total Neu5Ac were released using trichloroacetic acid and hydrochloric acid and purified using a HLB column. The linearity from 0.05 to 5.0 μg/mg Neu5Ac was adequate. Sialic acid recoveries ranged from 91.8% to 112.4%. The detection and quantification limits (limit of detection, 0.01 μg Neu5Ac/mg; limit of quantitation, 1.08 μg Neu5Ac/mg) were low enough to determine the sialic acid in infant formulas. The validated method is highly reproducible and sensitive, and it is easy to perform.