Development of a method employing reversed‐phase thin‐layer chromatography for establishing milk fat purity with respect to adulteration with vegetable oils. (28th October 2014)

• Record Type: Journal Article
• Authors: Upadhyay, Neelam; Kumar, Anil; Rathod, Gopal; Goyal, Ankit; Lal, Darshan
• Is Part Of: International journal of dairy technology. Volume 68:Number 2(2015:May)
• Page Start: 207
• Page End: 217
• Publication Date: 2014-10-28
• Subjects: Adulteration -- Milk fat -- Vegetable oils -- Unsaponifiable matter -- RP‐Thin‐Layer Chromatography -- β‐sitosterol; Dairying -- Periodicals; Dairying -- Great Britain -- Periodicals; 637
• DOI: 10.1111/1471-0307.12178
• Languages: English
• ISSNs: 1364-727X

Abstract

Abstract : A systematic study was conducted using reversed‐phase thin‐layer chromatography wherein unsaponifiable matter of samples namely, pure cow and buffalo milk fats, vegetable oils (groundnut oil, soya bean oil and sunflower oil) and milk fats adulterated with vegetable oils (≥1%) were run along with the reference standards using two new solvent systems to detect adulteration in milk fat. The results of the study revealed that adulteration at even a 1% level could easily be detected using this rapid, reliable and reproducible method based on the presence of β‐sitosterol as a marker and some additional spots ascribable to their occurrence in vegetable oils only.