Guar gum fiber increases suppressor of cytokine signaling‐1 expression via toll‐like receptor 2 and dectin‐1 pathways, regulating inflammatory response in small intestinal epithelial cells. Issue 10 (31st July 2017)

• Record Type: Journal Article
• Authors: Van Hung, Tran; Suzuki, Takuya
• Is Part Of: Molecular nutrition & food research. Volume 61:Issue 10(2017)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2017-07-31
• Subjects: Dectin‐1 -- Guar gum -- Interleukin‐8 -- Intestinal epithelium -- Suppressor of cytokine signaling‐1 -- Toll‐like receptor 2; Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.201700048
• Languages: English
• ISSNs: 1613-4125

Abstract

Abstract : Scope: Direct regulation of intestinal inflammation by intact dietary fibers is still unclear. Here, the anti‐inflammatory regulation by intact guar gum (GG) was investigated using mice and human intestinal Caco‐2 cells. Methods and results: Administration of dextran sodium sulfate (DSS) increased myeloperoxidase activity and CXC motif chemokine ligand2 (an IL‐8 homolog) expression in the small intestines of mice, while supplemental GG reduced these increases. Stimulation of Caco‐2 cells with tumor necrosis factor (TNF)‐α induced IL‐8 expression through nuclear factor kappa B p65, spleen tyrosine kinase, and mitogen‐activated protein kinases pathways. Pre‐treatment of cells with GG reduced the TNF‐α‐induced IL‐8 expression and cellular signaling. GG increased the suppressor of cytokine signaling (SOCS)‐1 expression in Caco‐2 cells, suggesting that this is one of the probable mechanisms involved in GG‐mediated anti‐inflammatory regulation. The anti‐inflammatory regulation and SOCS‐1 expression induced by GG were sensitive to neutralization of toll‐like receptor (TLR)2 and dectin‐1, and to inhibition of Janus kinase (JAK) and tyrosine kinase cSrc pathways. Finally, supplemental GG increased SOCS‐1 expression in the small intestines of both DSS‐administered and normal mice. Conclusion: Intact GG activates TLR2 and dectin‐1, and increases SOCS‐1 expression via JAK and cSrc pathways, resulting in anti‐inflammatory regulation in intestinal epithelium. Abstract : Regulation of intestinal inflammation by dietary guar gum (GG) fiber was examined. GG directly activates cell surface TLR2 and dectin‐1 and increases the SOCS‐1 expression via JAK and cSrc pathways in small intestinal epithelial cells. The SOCS‐1 expression increased by GG reduces the TNF‐α‐induced cellular signaling pathways, ERK1/2, NF‐κB, p38 MAPK, and JNK1/2, and IL‐8 expression. Anti‐inflammatory regulation by the intact GG may contribute to intestinal homeostasis.