Biotransformation and metabolism of three mulberry anthocyanin monomers by rat gut microflora. (15th December 2017)

• Record Type: Journal Article
• Authors: Chen, Yao; Li, Qian; Zhao, Ting; Zhang, Zhen; Mao, Guanghua; Feng, Weiwei; Wu, Xiangyang; Yang, Liuqing
• Is Part Of: Food chemistry. Volume 237(2017)
• Page Start: 887
• Page End: 894
• Publication Date: 2017-12-15
• Subjects: Anthocyanin monomers -- Metabolism -- Phenolic acid -- HPLC-ESI-MS/MS -- Gut microbiota; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2017.06.054
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Metabolism of three mulberry anthocyanin monomers by gut microflora was investigated. Characterization of metabolites was performed by HPLC-ESI-MS/MS. In vitro faecal fermentation generated a wide range of phenolic acids. A combination of conversion steps for metabolism of anthocyanins was presented. The differential metabolism of the three ACNs in fresh rat feces was investigated. Abstract: Anthocyanins (ACNs) are naturally occurring components of human diet. Evidence has accumulated regarding the positive association of their intake with chronic disease. Because microbiota has been considered as a metabolic organ, the bacterial-dependent metabolisms of three types of ACNs from mulberry fruits (cyanidin-3-glucoside (C3G), cyanidin-3-rutinoside (C3R), delphinidin-3-rutinoside (D3R)) during a simulation of large intestine conditions were investigated. ACNs and metabolites were analysed and characterized by high performance liquid chromatography-electrospray ionization-mass spectrum (HPLC-ESI-MS/MS). C3G disappeared after 6 h of metabolism, while C3R and D3R were no longer detected after 8 h. The metabolism of C3G and C3R mainly resulted in the formation of protocatechuic, vanillic, and p -coumaric acids, as well as 2, 4, 6-trihydroxybenzaldehyde, while the main metabolites of D3R were gallic acid, syringic acid and 2, 4, 6-trihydroxybenzaldehyde. This research indicated that the intake of ACNs may result in the appearance of specific metabolites that exert a protective effect in the host physiology.