An immunoaffinity column for the selective purification of 3-methyl-quinoxaline-2-carboxylic acid from swine tissues and its determination by high-performance liquid chromatography with ultraviolet detection and a colloidal gold-based immunochromatographic assay. (15th December 2017)

• Record Type: Journal Article
• Authors: Peng, Dapeng; Zhang, Xiya; Wang, Yulian; Pan, Yuanhu; Liu, Zhenli; Chen, Dongmei; Sheng, Feng; Yuan, Zonghui
• Is Part Of: Food chemistry. Volume 237(2017)
• Page Start: 290
• Page End: 296
• Publication Date: 2017-12-15
• Subjects: 3-Methyl-quinoxaline-2-carboxylic acid -- Immunoaffinity column purification -- High-performance liquid chromatography with ultraviolet detection -- Colloidal gold-based immunochromatographic assay -- Edible tissues; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2017.05.115
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: This is the first demonstration of the use of IAC for the clean-up of MQCA. Based on IAC purification procedures, HPLC-UV and GICA methods were developed. Compared with traditional SPE, IAC provides a good purification effect. This method is an environmentally friendly approach to control MQCA. Abstract: An immunoaffinity column (IAC) for the selective purification of 3-methyl-quinoxaline-2-carboxylic acid (MQCA) from porcine muscle and the liver as well as the methods for its determination by high-performance liquid chromatography with ultraviolet detection (HPLC-UV) and a colloidal gold-based immunochromatographic assay (GICA) were developed. This is the first study that describes a clean-up method that employs an IAC for the determination of MQCA. Using the HPLC-UV method, the limits of detection and limits of quantification for MQCA in different samples were 1.0–3.0 μg kg −1 and 4.0–10.0 μg kg −1, respectively. The average recoveries of MQCA that was spiked into samples at concentrations of 4.0–100 μg kg −1 were 80.1–87.7%, with relative standard deviations of less than 8.5%. Using the GICA method, the limits of detection for MQCA in different samples were 10 μg kg −1 and 50 μg kg −1 . No false negatives or false positives were observed.