A fluorescence polarization assay for bisphenol analogs in soybean oil using glucocorticoid receptor. Issue 9 (6th April 2017)

• Record Type: Journal Article
• Authors: Guan, Tianzhu; Sun, Yonghai; Yu, Hansong; Li, Tiezhu; Zhang, Jie; Zhang, Tiehua
• Is Part Of: European journal of lipid science and technology. Volume 119:Issue 9(2017)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2017-04-06
• Subjects: Bisphenol analogs -- Fluorescence polarization -- Glucocorticoid receptor -- Soybean oil; Oils and fats, Edible -- Periodicals; Lipids -- Periodicals; 660.63
• DOI: 10.1002/ejlt.201700042
• Languages: English
• ISSNs: 1438-7697

Abstract

Abstract : A fluorescence polarization (FP) assay based on receptor was developed for the determination of bisphenol analogs (BPs) in soybean oil. In the competitive binding system, dexamethasone fluorescein (Dex‐fl) and glucocorticoid receptor (GR) were selected as probe and recognition element, respectively. Crucial factors that affect the precision and accuracy were optimized. After development, the method was characterized by IC50 values ranging from 1.8 to 66.9 µM of 11 BPs. The established method revealed a limit of detection (LOD) of 1.0 µM and a working range of 7.6–30 µM for BPA in soybean oil. Analysis of spiked soybean oil samples showed that acceptable recoveries ranged from 71.99 to 82.95% with coefficients of variation (CVs) less than 10%. Furthermore, the results detected by the FP assay showed good agreement with that of HPLC with a fit of R 2 = 0.9171. It is concluded that this method offers a reliable alternative for determination of BPs in soybean oil. Practical applications: This work aims to develop FP assay for determination of BPs in soybean oil. Based on the recombinant GR, this method can detect multiple BPs simultaneously. It can potentially be used for high‐throughput screening of BPs, which may serve as an assistant of chromatographic techniques. Abstract : A fluorescence polarization (FP) assay i developed for determination of bisphenol analogs (BPs) in soybean oil. A recombinant glucocorticoid receptor (GR) and a fluorescein‐labeled dexamethasone derivative (Dex‐fl) are employed as recognition element and fluorescent tracer, respectively. This method can detect multiple BPs simultaneously and potentially be used for high‐throughput screening of BPs.