HPTLC methods for qualitative and quantitative analysis of selected furocoumarins in essential oils. (September 2017)

• Record Type: Journal Article
• Authors: Le Borgne, Erell; Cicchetti, Esméralda; Bertrand, Thomas
• Is Part Of: Flavour and fragrance journal. Volume 32:Number 5(2017:Sep.)
• Page Start: 330
• Page End: 339
• Publication Date: 2017-09
• Subjects: bergapten -- furocoumarins -- HPTLC; Flavor -- Periodicals; Odors -- Periodicals; Smell -- Periodicals; 668.54
• DOI: 10.1002/ffj.3394
• Languages: English
• ISSNs: 0882-5734

Abstract

Abstract: The concentration of furocoumarins, known to induce mutagenesis, carcinogenesis and photodermatitis under UV radiation, is limited by the EU cosmetic regulation in consumer products such as cosmetics, sun protection and bronzing formulations. The bergapten concentration is also limited by the International Fragrance Association. Analytical methods have been proposed for their quantification using high‐performance liquid chromatography (HPLC) coupled with UV detection or mass spectrometry with limits of detection (LOD) and quantification (LOQ) in accordance with the regulation. These methods are time and solvent consuming for routine quality control analyses. In this context, simple and fast HPLC methods in one‐ (1‐D) and two‐ (2‐D) dimensions were developed for the simultaneous analysis of the 15 most common furocoumarins reported in Citrus essential oils: psoralen, bergapten, xanthotoxin, isopimpinellin, oxypeucedanin, oxypeucedanin hydrate, byakangelicol, byakangelicin, heraclenin, 8‐geranyloxypsoralen, bergamottin, imperatorin, isoimperatorin, phellopterin, epoxybergamottin along with five other furocoumarins known to be present in Angelica, bergamot or Heracleum species: angelicin, bergaptol, isobergapten, psora‐4 and 5‐(4‐phenoxybutoxy)psoralen. The 1‐D method was validated for the quantification of bergapten using a Camag TLC Scanner as a detector. The LOD was determined at 2 mg/kg. The method was linear from 2 to 100 mg/kg and the method range was defined using accuracy profiles at with an 80% tolerance interval, with a 30% error, to be 5–100 mg/kg in acetonitrile and 13–100 mg/kg in matrix. Abstract : Two HPTLC methods were described for the analysis of the 20 most common furocoumarins found in essential oils. The method was applied for the determination of bergapten in accordance with regulated limits. The method was validated according to the accuracy profile methodology.