A membrane-based fluorescence-quenching immunochromatographic sensor for the rapid detection of tetrodotoxin. (November 2017)

• Record Type: Journal Article
• Authors: Shen, Haicong; Zhang, Shiwei; Fu, Qiangqiang; Xiao, Wei; Wang, Shifeng; Yu, Shiting; Xiao, Meng; Bian, Hongfen; Tang, Yong
• Is Part Of: Food control. Volume 81(2017:Nov.)
• Page Start: 101
• Page End: 106
• Publication Date: 2017-11
• Subjects: Tetrodotoxin -- Immunochromatographic sensor -- Fluorescence quenching -- Positive readout; FQ-ICS fluorescence-quenching immunochromatographic sensor -- TTX tetrodotoxin -- FRET fluorescence resonance energy transfer -- LOD detection limit -- DLR detection linear range -- LD50 median lethal dose -- MBA mouse bioassays -- LC-MS liquid chromatography-mass spectrometry -- SPR surface plasmon resonance -- ECL electrochemiluminescence sensor -- ELISA enzyme-linked immunosorbent assay -- LFICS lateral flow immunochromatographic sensors -- FQ-ICS fluorescence quenching immunochromatographic sensor -- FM Fluorescent microsphere -- AuNPs gold nanoparticles -- mAb monoclonal antibody -- AuNPs-mAb gold nanoparticles labeled mAb -- FM-BSA-TTX Fluorescent microsphere labeled BSA-TTX -- FM-BSA Fluorescent microsphere labeled BSA; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2017.06.001
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: A membrane-based fluorescence-quenching immunochromatographic sensor (FQ-ICS) was developed to detect tetrodotoxin (TTX) in as little as 12 min by utilizing fluorescence resonance energy transfer (FRET). Compared with traditional ICS for detecting TTX, this FQ-ICS provided a positive readout of the signal intensity as well as higher sensitivity. The signal detected during tests increased along with increasing TTX concentrations within a determinate concentration range. The limit of detection (LOD) of the method was 0.78 ng/mL, a value more than 20 times lower than the latest reported value using colloidal gold strips for TTX detection. Furthermore, the detection linear range (DLR) was 3.13–50 ng/mL. Recovery rates for the FQ-ICS used to detect TTX in spiked samples ranged from 61.3% to 70.4%. These results indicate that the FQ-ICS is highly sensitive and suitable for the rapid detection of TTX. Highlights: A fluorescence-quenching strip was applied to rapid, positive readout, and high sensitive tetrodotoxin detection. This strip provided a turn-on readout mode of fluorescence signal, which was more consumer-friendly. The visual detection limit of the strip was 20 times lower than colloidal gold strip for tetrodotoxin detection.