High Content Analysis technology for evaluating the joint toxicity of sunset yellow and sodium sulfite in vitro. (15th October 2017)

• Record Type: Journal Article
• Authors: Qu, Daofeng; Gu, Yanpei; Feng, Lifang; Han, Jianzhong
• Is Part Of: Food chemistry. Volume 233(2017)
• Page Start: 135
• Page End: 143
• Publication Date: 2017-10-15
• Subjects: HepG2 cell -- Food additives -- Joint toxicity -- HCA -- Synergistic joint action -- In vitro; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2017.04.102
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: The study investigated the toxicity of selected food additives and their damaging mechanisms of action. The High Content Analysis (HCA) showed that sunset yellow and sodium sulfite had a synergistic effect. HCA is more convenient to evaluate the effects of toxicity and mechanisms of action than conventional methods. Abstract: Foods contain various additives that affect our daily lives. At present, food additive safety evaluation standards are based on the toxicity of single additives, but food additives are often used in combination and may have additive, synergistic or antagonistic actions. The current study investigated the toxicity of food additives and mechanisms of damage in HepG2 cells using High Content Analysis (HCA). We used the CCK-8 assay to determine cell viability, providing an experimental basis for determining the safety of food additives. All of the food additives tested were observed to decrease the growth of HepG2 cells in a dose-dependent manner. Sunset yellow and sodium sulfite had IC50 values of 1.06, and 0.30 g/L at 24 h, respectively. HCA showed that both sunset yellow and sodium sulfite had synergistic effects on cell number, membrane permeability, mitochondrial membrane potential, intracellular calcium level, oxidative stress, and high dose group DNA damage.