Upconversion fluorescence imaging of HeLa cells using ROS generating SiO2-coated lanthanide-doped NaYF4 nanoconstructs. Issue 48 (12th June 2017)

• Record Type: Journal Article
• Authors: Kowalik, Przemysław; Elbaum, Danek; Mikulski, Jakub; Fronc, Krzysztof; Kamińska, Izabela; Morais, Paulo C.; Eduardo de Souza, Paulo; Nunes, Rodrigo Barbosa; Veiga-Souza, Fabiane Hiratsuka; Gruzeł, Grzegorz; Minikayev, Roman; Wojciechowski, Tomasz; Mosiniewicz-Szablewska, Ewa; Szewczyk, Maciej; Pawlyta, Mirosława; Sienkiewicz, Andrzej; Łapiński, Mariusz; Zajdel, Karolina; Stępień, Piotr; Szczepkowski, Jacek; Jastrzębski, Włodzimierz; Frontczak-Baniewicz, Małgorzata; Paszkowicz, Wojciech; Sikora, Bożena
• Is Part Of: RSC advances. Volume 7:Issue 48(2017)
• Page Start: 30262
• Page End: 30273
• Publication Date: 2017-06-12
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c6ra25383k
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Multicolor upconversion of SiO2 -coated nanoparticles using for cells imaging and reactive oxygen species generation. Abstract : Inorganic nanomaterials able to generate reactive oxygen species (ROS) are promising components for modern medical applications. Activated by near-infrared light, up-converting β-NaYF4 doped with Er 3+ –Yb 3+ and Tm 3+ –Yb 3+ pair ions nanoparticles (UCNPs), have a wide range of applications in biological imaging as compared to traditional reagents excited by ultra-violet or visible light. We analysed the green-red and the blue-red luminescence to explain the mechanism of the upconversion depended on the surface condition. The influence of SiO2 coating on the cytotoxicity of the as-produced UCNPs towards HeLa cancer cells was reported. We demonstrated a possibility of a direct UCNPs application to photodynamic therapy, without need to attach additional molecules to their surface. The presence of Tm 3+ –Yb 3+ pair ions, thus ROS generation capability, renders the SiO2 shell coated nanoparticles to become potentially useful theranostic agent.