Upconversion fluorescence imaging of HeLa cells using ROS generating SiO2-coated lanthanide-doped NaYF4 nanoconstructs. Issue 48 (12th June 2017) - Kowalik, Przemysław
Abstract : Multicolor upconversion of SiO2 -coated nanoparticles using for cells imaging and reactive oxygen species generation. Abstract : Inorganic nanomaterials able to generate reactive oxygen species (ROS) are promising components for modern medical applications. Activated by near-infrared light, up-converting β-NaYF4 doped with Er 3+ –Yb 3+ and Tm 3+ –Yb 3+ pair ions nanoparticles (UCNPs), have a wide range of applications in biological imaging as compared to traditional reagents excited by ultra-violet or visible light. We analysed the green-red and the blue-red luminescence to explain the mechanism of the upconversion depended on the surface condition. The influence of SiO2 coating on the cytotoxicity of the as-produced UCNPs towards HeLa cancer cells was reported. We demonstrated a possibility of a direct UCNPs application to photodynamic therapy, without need to attach additional molecules to their surface. The presence of Tm 3+ –Yb 3+ pair ions, thus ROS generation capability, renders the SiO2 shell coated nanoparticles to become potentially useful theranostic agent.