An ultrasensitive gray-imaging-based quantitative immunochromatographic detection method for fumonisin B1 in agricultural products. (October 2017)

• Record Type: Journal Article
• Authors: Tang, Xiaoqian; Li, Peiwu; Zhang, Zhaowei; Zhang, Qi; Guo, Jingnan; Zhang, Wen
• Is Part Of: Food control. Volume 80(2017:Oct.)
• Page Start: 333
• Page End: 340
• Publication Date: 2017-10
• Subjects: Fumonisin B1 -- Monoclonal antibody -- Gray imaging -- Immunoassay -- Agricultural product; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2017.05.013
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: Fumonisins (FBs) are widely found in rice, maize, peanut, wheat, and other agricultural products. These have been detected using a chromatography technique, whereas the rapid assay by a highly sensitive monoclonal antibody is minimally reported. Herein, a highly sensitive monoclonal antibody (7A11) was successfully developed by hybridoma technique. The 50% inhibition concentration (IC50 ) of 7A11 monoclonal antibody was 0.32 ng/mL in an optimized buffer. The cross-reactivity between fumonisin B1 and fumonisin B2, B3 was 4.3% and 12.8%, respectively. Based on the newly developed 7A11 antibody, a high sensitivity indirect competitive enzyme-linked immunosorbent assay (icELISA) and gold nanoparticles-based gray imaging quantification immunoassay (GNPs-GI) were established. The analytical range of icELISA was 0.08–1.38 ng/mL and that for GNPs-GI was 0.24–15 ng/mL. Both the methods showed adequate recoveries (80.0–105.8% for icELISA and 78.5–115.2% for GNPs-GI) in spiked samples. Compared to high-performance liquid chromatography (HPLC), icELISA and GNPs-GI indicated reliability that could be used for further detection of fumonisin B1 in agricultural products. Highlights: A high sensitive novel monoclonal antibody against fumonisin B1 was achieved. A indirect competitive enzyme-linked immunosorbent assay (icELISA) was established for fumonisin B1 detection. A gold nanoparticles-based gray imaging quantification immunoassay (GNPs-GI) was developed for fumonisin B1 detection. The icELISA and GNPs-GI performed rapidly assay for fumonisin B1 in agricultural products.