Purification, characterization and bioactivities of polysaccharides from Pleurotus ferulae. Issue 5 (24th April 2017)

• Record Type: Journal Article
• Authors: Li, Jinyu; Yuan, Pengfei; Wang, Xinhui; Aipire, Adila; Li, Minjing; Yang, Jie; Tao, Hong; Ying, Tianlei; Fu, Changshuang; Wei, Xianxian; Zhang, Fuchun; Li, Jinyao
• Is Part Of: Food & function. Volume 8:Issue 5(2017)
• Page Start: 1905
• Page End: 1914
• Publication Date: 2017-04-24
• Subjects: Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Nutrition -- Periodicals; 664.07
• DOI: 10.1039/c7fo00227k
• Languages: English
• ISSNs: 2042-6496

Abstract

Abstract : Pleurotus ferulae polysaccharides promote the maturation of dendritic cells. Abstract : Pleurotus ferulae is an edible mushroom and has been used in Uygur medicine for a long time. In this study, we purified polysaccharides from P. ferulae (PFPS) and investigated its structural characteristics. We obtained a homogeneous PFPS with a molecular weight of around 1600 kDa and prominent characteristic polysaccharide groups, which mainly contained glucose (97%), followed by mannose and galactose (3%). Both 1 H and 13 C NMR spectra indicated that PFPS contained both α- and β-anomeric configurations. Atomic force microscopy and Congo red-staining data further suggested that PFPS belonged to a linear branched structure that existed in flexible single chains at low concentrations and could form aggregates such as a triple-helical structure at high concentrations. Moreover, PFPS promoted the maturation of dendritic cells through a TLR4 mediated signaling pathway, which is characterized by the increased expressions of CD40, CD86, IL-12 and TNF-α and the decreased endocytosis. The results suggest that PFPS has immunoregulatory activities.