Fabrication of nanoemulsion-filled alginate hydrogel to control the digestion behavior of hydrophobic nobiletin. (1st September 2017)

• Record Type: Journal Article
• Authors: Lei, Lingling; Zhang, Yazhen; He, Lingling; Wu, Shan; Li, Bin; Li, Yan
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 82(2017)
• Page Start: 260
• Page End: 267
• Publication Date: 2017-09-01
• Subjects: Emulsion -- Alginate hydrogel -- Polymethoxylated flavone -- Digestion precipitation -- Bioaccessibility; Nobiletin (PubChem CID:72344) -- Tween 80 (PubChem CID:6364656) -- Span 20(PubChem CID:14926) -- Sodium alginate (PubChem CID:91666323) -- D-glucono-δ-lactone (PubChem CID:763) -- Dimethyl sulfoxide (PubChem CID:679) -- Calcium chloride (PubChem CID:5284359) -- Sodium chloride (PubChem CID:5324) -- Lipase (PubChem CID:54603431); Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2017.04.051
• Languages: English
• ISSNs: 0023-6438

Abstract

Abstract: Precipitation of hydrophobic bioactive compounds during gastrointestinal (GI) tract usually causes their low absorption and bioaccessibility. In the present work, alginate hydrogel was utilized to encapsulate nobiletin-loaded nanoemulsion droplets, aiming to control the crystallization and release rate of nobiletin. Images by Scanning Electronic Microscopy displayed that nanoemulsion droplets were entrapped in the network of alginate hydrogel matrix. X-ray diffraction results demonstrated that crystallinity of nobiletin in the hydrogel matrix was partially retarded, where nobiletin crystals were softer than those in nanoemulsions. In vitro release rate of nobiletin from hydrogel was much higher at pH 7.4 (>80%) than that at pH 1.2 (<27%). In vitro digestion experimental results revealed that the lipolysis rate of nanoemulsions in the hydrogel was lower than that of bulk nanoemulsions. No nobiletin crystal was formed as hydrogel passed through GI tract. In comparison, many nobiletin crystals were observed in the nanoemulsion systems. The bioaccessibility of nobiletin was determined to be lower in alginate hydrogel ( i.e. 44.7± 0.4% at 4.5 mg/mL) than that in nanoemulsions ( i.e. 67.2± 0.4% at 4.5 mg/mL). It concluded that nanoemulsion-filled hydrogel could achieve the sustained release and absorption of nobiletin and prevent its precipitation in the GI tract. Highlights: Entrapment of alginate hydrogel enhanced stability of nobiletin and nanoemulsion. Alginate hydrogel matrix controlled the crystalline property of nobiletin. Filled-hydrogel prevented the formation of nobiletin precipitation during digestion.