Development of a competitive immunochromatographic assay for the sensitive detection of amantadine in chicken muscle. (1st October 2017)

• Record Type: Journal Article
• Authors: Wu, Songsong; Zhu, Fangfei; Hu, Liming; Xia, Jun; Xu, Guomao; Liu, Daofeng; Guo, Qi; Luo, Kai; Lai, Weihua
• Is Part Of: Food chemistry. Volume 232(2017)
• Page Start: 770
• Page End: 776
• Publication Date: 2017-10-01
• Subjects: Amantadine -- Immunochromatography -- Semi-quantitative analysis -- Chicken muscle; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2017.04.058
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: CGICA was established for the detection of AMD in chicken muscle. CGICA had high sensitivity at 1.80 ng/mL. The CGICA results were highly correlated with the ELISA and UPLC–MS/MS results. CGICA is suitable for the rapid detection of AMD in chicken muscle on-site. Abstract: Amantadine (AMD) is a prohibitive veterinary medicine in the entire world. In this study, a sensitive colloidal gold immunochromatographic assay (CGICA) was established for the rapid semi-quantitative detection of AMD in chicken muscle. Under optimal conditions, the detection results were obtained in 12 min with a limit of detection for 1.80 ng/mL. CGICA presented a good linear range from 2.5 ng/mL to 25 ng/mL, with only 11.5% cross-reactivity with rimantadine. The recovery rates for the fortified samples were ranged from 81% to 120%. The coefficient of variation of the intra-assay and inter-assay was less than 15%. The accuracy of CGICA was confirmed by systematically comparing the result of the proposed method with enzyme-linked immunosorbent assay and ultra-high-performance liquid chromatography–tandem mass spectrometry. Given the advantages of its simplicity, convenience, and speediness, the proposed CGICA is suitable for the on-site rapid detection of AMD in chicken muscle.