Effect of complexes of cyanidin-3-diglucoside-5-glucoside with rutin and metal ions on their antioxidant activities. (1st October 2017)

• Record Type: Journal Article
• Authors: Qian, Bing-Jun; Wu, Chou-Fei; Lu, Man-Man; Xu, Wei; Jing, Pu
• Is Part Of: Food chemistry. Volume 232(2017)
• Page Start: 545
• Page End: 551
• Publication Date: 2017-10-01
• Subjects: Anthocyanins -- Cyanidin-3-diglucoside-5-glucoside -- Complexes -- Copigmentations -- Antioxidant activity -- Molecular interaction; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2017.04.010
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: CY3D5G-rutin-Fe(III) showed supramolecular properties clearly via visible, CD and FTIR spectra. CY3D5G-rutin-Mg(II)/Fe(III) showed synergistic interactions on ROS reduction in ORAC assay. CY3D5G-rutin-Mg(II)/Fe(III) were synergistic/additive interactions on cellular ROS levels. The molecular antioxidant properties were affected by the combination of complexes. Fe(III) might exhibit a negative effect on cellular ROS reduction. Abstract: Studies have enabled a molecular understanding of the anthocyanin copigmentation phenomenon over several decades. However, the effect of combinations of, or even supramolecular assemblies of, anthocyanins with other phenols and/or metal ions on their antioxidative activity was unclear. In this study, anthocyanin complexes of cyanidin-3-diglucoside-5-glucoside (CY3D5G), rutin and Mg(II)/Fe(III) were constructed, analyzed, and evaluated for their antioxidant effects. The CY3D5G-rutin-Fe(III) exhibited supramolecular properties via visible, CD and FTIR spectra among complexes. The interaction of CY3D5G-rutin, CY3D5G-rutin-Mg(II), or CY3D5G-rutin-Fe(III) was synergistic ( P < 0.05) in the ORAC assay. On cellular ROS levels, the median effective concentration of the CY3D5G-rutin-Mg(II) was 7.76 μmol QE/L and exhibited a synergistic interaction (CI = 0.67, P < 0.05), whereas the CY3D5G-rutin-Fe(III) (CI = 0.79, P = 0.074) was additive. The results indicate that the antioxidant properties were affected by the molecular combination. Additionally, Fe(III) might exhibit a negative effect, since the CY3D5G-Fe(III) required a greater concentration than CY3D5G to achieve the same effect on cells.