Isotope dilution HPLC-MS/MS for simultaneous quantification of acrylamide and 5-hydroxymethylfurfural (HMF) in thermally processed seafood. (1st October 2017)

• Record Type: Journal Article
• Authors: Qin, Lei; Zhang, Yu-Ying; Xu, Xian-Bing; Wang, Xu-Song; Liu, Hong-Wei; Zhou, Da-Yong; Zhu, Bei-Wei; Thornton, Megan
• Is Part Of: Food chemistry. Volume 232(2017)
• Page Start: 633
• Page End: 638
• Publication Date: 2017-10-01
• Subjects: Acrylamide -- 5-Hydroxymethylfurfural -- Seafood -- HPLC-MS/MS -- Isotope dilution; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2017.04.069
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: A revised method for the simultaneous quantitation of acrylamide and HMF in seafood is described. Good quantification accuracy, precision, and recovery were achieved. The acrylamide and HMF content of 14 commercial seafood samples was determined using this method. Abstract: The objective of this study was to develop an accurate, robust and rapid HPLC-MS/MS-based method capable of quantifying acrylamide and 5-hydroxymethylfurfural (HMF) simultaneously. A Phenomenex Synergi Fusion-RP C18 column (50 mm × 2.5 mm, 2 μm) was used, and the MS/MS instrument was operated in multiple reaction monitoring mode. The isotope dilution method was used to correct for the matrix effect from samples. This method demonstrated low limits of quantification (2.12 ng/mL for acrylamide and 4.86 ng/mL for HMF) and excellent linearity (R 2 > 0.999). This method also demonstrated excellent quantification accuracy, precision, and recovery (87–110%). Using the method developed, target analytes were quantified in 14 thermally-processed seafood samples. Resulting concentrations ranged from 5.58 to 50.35 μg/kg for acrylamide, and from 12.54 to 2276.44 μg/kg for HMF. The proposed isotope dilution HPLC-MS/MS method is a valid and rapid technique for simultaneous analysis of acrylamide and HMF in seafood.