Physicochemical properties and cellular protection against oxidation of degraded Konjac glucomannan prepared by γ-irradiation. (15th September 2017)

• Record Type: Journal Article
• Authors: Jian, Wenjie; Tu, Liying; Wu, Lanlan; Xiong, Hejian; Pang, Jie; Sun, Yuan-ming
• Is Part Of: Food chemistry. Volume 231(2017)
• Page Start: 42
• Page End: 50
• Publication Date: 2017-09-15
• Subjects: Sodium nitrate (PubChem CID: 24268) -- Sodium chloride (PubChem CID: 5234) -- Sodium azide (PubChem CID: 33557) -- Ethanol (PubChem CID: 702) -- Penicillin (PubChem CID: 5904) -- Streptomycin (PubChem CID: 19649) -- Hydrochloric acid (PubChem CID: 313) -- Sulphuric acid (PubChem CID: 1118) -- Konjac glucomanan (PubChem CID: 24892726); Konjac glucomannan -- Dispersibility -- Degradation -- γ-Irradiation -- Physicochemical properties -- Antioxidation -- LO2 cell; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2017.03.121
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Irradiation in dosage of 100 kGy leads to excellent dispersibility in KGM. 100 kGy-KGM demonstrates excellent protective effect against oxidative damage. Possible mechanism for anti-oxidative activity in 100 kGy-KGM was analyzed. Irradiation was proven an effective method to improve properties of KGM. Abstract: Konjac glucomannan (KGM) is an important functional polysaccharide in food research. However, unstable dispersibility of KGM inhibits its in-depth study and wide application. In this study, a degraded KGM (100 kGy-KGM), which showed excellent dispersibility and specific physicochemical properties, were obtained by γ-irradiation in a dosage of 100 kGy. We investigated the protective effect of 100 kGy-KGM against H2 O2 induced oxidative damage in LO2 cells. Our results demonstrated that pretreatment of LO2 cells with 100 kGy-KGM not only significantly increased cellular survivals and activities of GSH-Px and CAT, but also reduced levels of LDH, MDA and intracellular accumulation of ROS. The marked protective effect against oxidative damage and excellent dispersibility in 100 kGy-KGM allowed its possible use as an antioxidant. Our study provided fundamental knowledge to understand the structure-functions relationships of degraded-KGM, which could result in a theoretical guidance for the future application of KGM.