Facile preparation of magnetic carbon nanotubes-immobilized lipase for highly efficient synthesis of 1, 3-dioleoyl-2-palmitoylglycerol-rich human milk fat substitutes. (1st August 2017)

• Record Type: Journal Article
• Authors: Zheng, Mingming; Wang, Shi; Xiang, Xia; Shi, Jie; Huang, Juan; Deng, Qianchun; Huang, Fenghong; Xiao, Junyong
• Is Part Of: Food chemistry. Volume 228(2017)
• Page Start: 476
• Page End: 483
• Publication Date: 2017-08-01
• Subjects: 1, 3-Dioleoyl-2-palmitoylglycerol -- Structured lipid -- Magnetic carbon nanotubes -- Enzymatic acidolysis; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2017.01.129
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: A novel magnetically retrievable immobilized lipase was prepared. OPO-rich TAGs was efficiently synthesized by enzymatic acidolysis. The activity and reusability of CLL @mMWCNTs was superior to Lipozyme RM IM. Production of OPO-rich TAGs was performed using lard and restructured palm oil. Abstract: In this study, Candida lipolytica lipase ( CLL ) was immobilized on magnetic multi-walled carbon nanotubes (mMWCNTs) via hydrophobic and cation-exchange interaction. The resultant immobilized CLL showed much better thermal stability, biocatalyst activity and easier recycling than the free form. A method for efficient enzymatic acidolysis of tripalmitin (PPP) with oleic acid (OA), to produce OPO-rich TAGs, was developed, using the immobilized CLL as the biocatalyst. Under optimized conditions (2% water, 20 mg/ml enzyme, 1:6 PPP/OA, 50 °C, 2 h), the content of OPO in the final product reached 46.5%. CLL @mMWCNTs had a better activity and manipulative stability than commercial lipases. More importantly, the feasibility of CLL @mMWCNTs was also validated in the practical production of OPO-rich TAGs, using lard and restructured palm oil as the raw material. These results suggest that CLL @mMWCNTs is a promising biocatalyst for the OPO-rich TAGs production and will be helpful for the infant formula industry.