Structural characterization and immunostimulatory activity of a glucan from natural Cordyceps sinensis. (June 2017)

• Record Type: Journal Article
• Authors: Wang, Junqiao; Nie, Shaoping; Cui, Steve W.; Wang, Zhijun; Phillips, Aled O.; Phillips, Glyn O.; Li, Yajing; Xie, Mingyong
• Is Part Of: Food hydrocolloids. Volume 67(2017)
• Page Start: 139
• Page End: 147
• Publication Date: 2017-06
• Subjects: Natural Cordyceps sinensis -- α-glucan -- Caterpillar fungus -- Immunostimulatory; Hydrocolloids -- Periodicals; Food additives -- Periodicals; Colloïdes -- Périodiques; Aliments -- Additifs -- Périodiques; Colloids; Food additives; Periodicals; Electronic journals; 664.06
• DOI: 10.1016/j.foodhyd.2017.01.010
• Languages: English
• ISSNs: 0268-005X

Abstract

Abstract: A water-soluble polysaccharide, named NCSP-50, was obtained from natural Cordyceps sinensis by hot water extraction and ethanol fractionation precipitation. It was eluted as a single symmetrical peak and had an average molecular weight of 9.76 × 10 5 Da. The structure was determined by monosaccharide composition, methylation analysis, 1D/2D NMR spectroscopy, and enzymatic hydrolysis and characterization of the oligosaccharides by MALDI-TOF mass spectrometry. The repeating unit of this polysaccharide was proposed as follows: This glucan showed potent immunostimulatory activity on the basis of its significant abilities to promote macrophage proliferation, enhance NO production, as well as and cytokines (IL-1β and TNF-α) secretion. Graphical abstract: Highlights: A homogenous polysaccharide (NCSP-50) from natural Cordyceps sinensis was isolated and purified. NCSP-50 is a 4-linked α-glucan substituted by α-glucose units attached to C-6. The glucan showed a significant immunostimulatory effect on macrophages.