Grape marc as a source of carbohydrates for bioethanol: Chemical composition, pre-treatment and saccharification. (October 2015)

• Record Type: Journal Article
• Authors: Corbin, Kendall R.; Hsieh, Yves S.Y.; Betts, Natalie S.; Byrt, Caitlin S.; Henderson, Marilyn; Stork, Jozsef; DeBolt, Seth; Fincher, Geoffrey B.; Burton, Rachel A.
• Is Part Of: Bioresource technology. Volume 193(2015)
• Page Start: 76
• Page End: 83
• Publication Date: 2015-10
• Subjects: A:X arabinose:xylose ratio -- AIR alcohol-insoluble residue -- Ara arabinose -- ASE accelerated solvent extractor -- CDTA cyclohexane-1, 2-diamine tetraacetate -- Fuc fucose -- Gal galactose -- GalA galacturonic acid -- Glc glucose -- GlcA glucuronic acid -- HILIC hydrophilic interaction chromatography -- HMF 5-(hydroxymethyl)furfural -- HPLC high-performance liquid chromatography -- Man mannose -- mol% relative percent molarity -- MS mass spectrometry -- Na2CO3 sodium carbonate -- NCPs noncellulosic polysaccharides -- Rha rhamnose -- WSC water soluble carbohydrates -- Xyl xylose; Bioethanol -- Grape marc -- Polysaccharide -- Pre-treatment -- Saccharification; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2015.06.030
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: The chemical composition of white and red grape marc was determined. Marc carbohydrates were characterized using enzyme digests, HPLC and MALDI-TOF-MS. Dilute acid pre-treatments liberated glucose more efficiently than thermal treatments. White marc contains 40% fermentable water-soluble carbohydrates. Theoretical ethanol yields were calculated based on chemical composition. Abstract: Global grape production could generate up to 13 Mt/yr of wasted biomass. The compositions of Cabernet Sauvignon (red marc) and Sauvignon Blanc (white marc) were analyzed with a view to using marc as raw material for biofuel production. On a dry weight basis, 31–54% w/w of the grape marc consisted of carbohydrate, of which 47–80% was soluble in aqueous media. Ethanol insoluble residues consisted mainly of polyphenols, pectic polysaccharides, heteroxylans and cellulose. Acid and thermal pre-treatments were investigated for their effects on subsequent cellulose saccharification. A 0.5 M sulfuric acid pre-treatment yielded a 10% increase in the amount of liberated glucose after enzymatic saccharification. The theoretical amount of bioethanol that could be produced by fermentation of grape marc was up to 400 L/t. However, bioethanol from only soluble carbohydrates could yield 270 L/t, leaving a polyphenol enriched fraction that may be used in animal feed or as fertilizer.