The small molecule probe PT-Yellow labels the renal proximal tubules in zebrafish. Issue 2 (18th November 2014)

• Record Type: Journal Article
• Authors: Sander, Veronika; Patke, Shantanu; Sahu, Srikanta; Teoh, Chai Lean; Peng, Zhenzhen; Chang, Young-Tae; Davidson, Alan J.
• Is Part Of: Chemical communications. Volume 51:Issue 2(2015)
• Page Start: 395
• Page End: 398
• Publication Date: 2014-11-18
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c4cc08075k
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : The fluorescent compound PT-Yellow is a novel tool to label the proximal tubule cells of the zebrafish kidney in vivo . Abstract : We report the development of a small fluorescent molecule, BDNCA3-D2, herein referred to as PT-Yellow. Soaking zebrafish embryos in PT-Yellow or intraperitoneal injection into adults results in non-toxic in vivo fluorescent labeling of the renal proximal tubules, the major site of blood filtrate reabsorption and a common target of injury in acute kidney injury. We demonstrate the applicability of this new compound as a rapid and simple readout for zebrafish kidney filtration and proximal tubule reabsorption function.