The small molecule probe PT-Yellow labels the renal proximal tubules in zebrafish. Issue 2 (18th November 2014) - Sander, Veronika
Abstract : The fluorescent compound PT-Yellow is a novel tool to label the proximal tubule cells of the zebrafish kidney in vivo . Abstract : We report the development of a small fluorescent molecule, BDNCA3-D2, herein referred to as PT-Yellow. Soaking zebrafish embryos in PT-Yellow or intraperitoneal injection into adults results in non-toxic in vivo fluorescent labeling of the renal proximal tubules, the major site of blood filtrate reabsorption and a common target of injury in acute kidney injury. We demonstrate the applicability of this new compound as a rapid and simple readout for zebrafish kidney filtration and proximal tubule reabsorption function.