Anti-inflammatory effects of Dioscorea alata L. anthocyanins in a TNBS-induced colitis model. Issue 2 (25th January 2017)

• Record Type: Journal Article
• Authors: Chen, Tao; Hu, Shihui; Zhang, Haiwen; Guan, Qingfeng; Yang, Yuhui; Wang, Xuemei
• Is Part Of: Food & function. Volume 8:Issue 2(2017)
• Page Start: 659
• Page End: 669
• Publication Date: 2017-01-25
• Subjects: Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Nutrition -- Periodicals; 664.07
• DOI: 10.1039/c6fo01273f
• Languages: English
• ISSNs: 2042-6496

Abstract

Abstract : DACNs isolated from the purple tubers of Dioscorea alata L., showed significant anti-inflammatory activity in TNBS-induced colitis in a mice model. Abstract : The purple yam, Dioscorea alata L., is an important source of starch, vitamins and polyphenols. Five different pigments from the purple tubers of this plant were separated by high-performance liquid chromatography–mass spectrometry, and the anthocyanin fraction (DACN) was collected. The anti-inflammatory effects of DACNs were investigated at different concentrations and compared with the standard colitis treatment, 5-aminosalicylic acid, in a trinitrobenzenesulfonic acid (TNBS)-induced colitis mouse model. Macro- and microscopic parameters including body weight change, disease activity index (DAI) and intestinal histology were used for the determination of the anti-inflammatory effects of DACNs. The gene expression levels of tight junction-related proteins in the intestine, myeloperoxidase activity, inducible nitric oxide synthase activity in colonic tissues and pro-inflammatory cytokine production in serum were also measured to elucidate the mechanism of DACN action. Eighty micrograms of DACNs per kilogram of body weight produced potent anti-inflammatory effects in the mouse model of inflammatory bowel disease (IBD), as shown by the DAI (2.78 ± 0.38 vs. 0.44 ± 0.51). Therefore, DACNs may be applied as a potential food supplement in IBD therapy.