The chemical profiling of loquat leaf extract by HPLC-DAD-ESI-MS and its effects on hyperlipidemia and hyperglycemia in rats induced by a high-fat and fructose diet. Issue 2 (26th January 2017)

• Record Type: Journal Article
• Authors: Chen, Bo; Long, Piaopiao; Sun, Yue; Meng, Qilu; Liu, Xingxun; Cui, Huanhuan; Lv, Qingya; Zhang, Liang
• Is Part Of: Food & function. Volume 8:Issue 2(2017)
• Page Start: 687
• Page End: 694
• Publication Date: 2017-01-26
• Subjects: Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Nutrition -- Periodicals; 664.07
• DOI: 10.1039/c6fo01578f
• Languages: English
• ISSNs: 2042-6496

Abstract

Abstract : The inhibitory effects of loquat leaf extract (LLE) on pancreatic α-amylase, α-glucosidase and hyperlipidemia were evaluated. Abstract : In this study, the inhibitory effects of loquat leaf extract (LLE) on pancreatic α-amylase and α-glucosidase, and the preventative effects of LLE on hyperlipidemia and hyperglycemia in rats induced by a high fat and fructose diet have been evaluated. The LLE was chemically described using a high performance liquid chromatography-diode array detector coupled with a mass spectrometer (HPLC-DAD-MS/MS). 20 compounds including phenolic acids, flavonoids and triterpene acids were tentatively identified with authentic compounds or by referring to published articles and accessible databases ( e.g. MassBank, METLIN). Enzyme activity measurements showed that the IC50 values of the LLE on α-amylase and α-glucosidase were 11.34 ± 1.04 mg mL −1 and 50.77 ± 1.04 μg mL −1, respectively. The calculated Michaelis–Menten constants indicated that the LLE is an effective inhibitor against α-glucosidase in a mixed-model competitive mode. The fluorescence data revealed that the LLE binds with α-amylase and α-glucosidase. The animal experiment results indicated that the LLE significantly decreased the levels of fasting blood glucose, and hepatic and serum triglycerides.