A novel ionic liquid-stable halophilic endoglucanase from Stachybotrys microspora. (March 2017)

• Record Type: Journal Article
• Authors: Ben Hmad, Ines; Boudabbous, Manel; Belghith, Hafedh; Gargouri, Ali
• Is Part Of: Process biochemistry. Volume 54(2017)
• Page Start: 59
• Page End: 66
• Publication Date: 2017-03
• Subjects: Stachybotrys microspora -- Halophilic endoglucanase -- Ionic liquid -- Purification -- Solvent tolerance; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2017.01.007
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Purification and biochemical characterization of endoglucanase from Stachybotrys microspora . EG1 is alkalitolerant, halophilic that is stimulated by 5 M NaCl. High ionic liquid-tolerant purified endoglucanase. EG1 is resisted to SDS and commercial detergents. Abstract: In this study, we isolated a novel endoglucanase (EG1) that exhibits optimal activity at pH 7 and 50 °C and retained >85% of its activity between pH 5 and 9 and >80% at 60 °C. In addition to its alkali tolerance, this EG1 is a halophilic enzyme as its activity was enhanced to 152% in the presence of 5 M NaCl. In this condition, optimal activity was observed at pH 8 and 70 °C. More interestingly, EG1 was active in the presence of an ionic liquid (IL) (100% and 80% activity in 5% and 10% [Bmim]Cl, respectively). Furthermore, it was extremely stable in the presence of various detergents, especially SDS (10%), which is a very rare property amongst fungal cellulases. All these properties make this enzyme suitable for many applications, mainly in biofuel, textile and detergent industries.