HPLC Analysis of Twelve Compounds from Artemisia sacrorum Ledeb and their Structural Elucidation. Issue 1 (18th May 2016)

• Record Type: Journal Article
• Authors: Bao, Wugedunqiqige; Wang, Qinghu; Han, Jingjing; Han, Narenchaoketu
• Is Part Of: Journal of food biochemistry. Volume 41:Issue 1(2017:Feb.)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2016-05-18
• Subjects: Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Biochemistry -- Periodicals; 664.024
• DOI: 10.1111/jfbc.12291
• Languages: English
• ISSNs: 0145-8884

Abstract

Abstract: An investigation of ethyl acetate extracts from Artemisa sacrorum Ledeb ( A. sacrorum ) has led to the isolation of two new flavonoids together with 10 known compounds. At the same time, a high‐performance liquid chromatographic method is described for the simultaneous determination of the 12 compounds in A. sacrorum . The separation by gradient elution was performed on Hypersil ODS‐2 column (250 mm × 4.6 mm, 5 μm) at 30C with acetonitrile and water as the mobile phase, and monitored by absorbance at 261 nm. The recovery of the method is from 93.33 to 98.67%, and linearity ( r > 0.9992) was obtained for 12 compounds. A high degree of specificity as well as repeatability and reproducibility (relative standard deviation values less than 2.0%) were also achieved. This assay was successfully applied to the determination of 12 compounds in 10 samples. Practical Applications: The antitumor effects of A. sacrorum have been well known in folk medicine, which suggesting that extracted components from A. sacrorum could potentially treat tumor disease. Therefore, a detailed analysis and recognition of structural compounds diversity in A. sacrorum is of great importance to evaluate their antitumor effects.