Enzymatic synthesis of lysophosphatidylcholine with n−3 polyunsaturated fatty acid from sn-glycero-3-phosphatidylcholine in a solvent-free system. (1st July 2017)

• Record Type: Journal Article
• Authors: Liu, Yanjun; Zhang, Qin; Guo, Yongli; Liu, Junyi; Xu, Jie; Li, Zhaojie; Wang, Jingfeng; Wang, Yuming; Xue, Changhu
• Is Part Of: Food chemistry. Volume 226(2017)
• Page Start: 165
• Page End: 170
• Publication Date: 2017-07-01
• Subjects: n−3 polyunsaturated fatty acids -- Lipozyme TL IM -- Lysophosphatidylcholine -- Docosahexaenoic acid -- sn-Glycero-3-phosphatidylcholine; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2017.01.066
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Lipases TLIM was more effective than phospholipases and other lipases for esterification of GPC and n −3 PUFA. Optimal conditions were a temperature of 45 °C, a mole ratio of 1:20 (GPC to n −3 PUFA) under vacuum for 64 h. The 1-acyl-sn-glycero-3-lysophosphatidylcholine (2-LPC) was predominant in the reaction. Abstract: The n −3 polyunsaturated fatty acids (PUFA)-rich lysophosphatidylcholine (LPC) was successfully synthesized by Thermomyces lanuginosus lipase (TL IM)-catalyzed esterification of glycerylphosphorylcholine (GPC) and n −3 PUFA-rich fatty acids in a solvent-free system. Effects of reaction temperature, enzyme loading and substrate mole ratio on the yield of LPC and incorporation of n −3 PUFA were evaluated. The acyl-specificities of five enzymes were tested for direct esterification of n −3 PUFA, and Lipozyme TL IM was found to be more effective than others for production of LPC with n −3 PUFA. Substrate mole ratio and reaction temperature, however, had no significant effect on the incorporation. The maximal yield of LPC was obtained under the following conditions: temperature 45 °C, enzyme loading 15% by weight and substrate mole ratio (GPC/ n −3 PUFA) 1:20. Furthermore, the composition of products were further investigated in the study. The 1-acyl-sn-glycero-3-lysophosphatidylcholine (2-LPC) was predominant in the mixtures at early stages of reaction, whereas less increment of 2-acyl-sn-glycero-3-lysophosphatidylcholine (1-LPC) and PC was observed at later stages.