Anti-apoptotic and anti-glycative effects of asiatic acid in the brain of d-galactose treated mice. Issue 2 (15th December 2014)

• Record Type: Journal Article
• Authors: Chao, Pei-chun; Yin, Mei-chin; Mong, Mei-chin
• Is Part Of: Food & function. Volume 6:Issue 2(2015)
• Page Start: 542
• Page End: 548
• Publication Date: 2014-12-15
• Subjects: Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Nutrition -- Periodicals; 664.07
• DOI: 10.1039/c4fo00862f
• Languages: English
• ISSNs: 2042-6496

Abstract

Abstract : Protection of asiatic acid (AA) in mice brain againstd -galactose (DG) induced aging was examined. Abstract : Protection of asiatic acid (AA) in mice brain againstd -galactose (DG) induced aging was examined. AA at 5, 10 or 20 mg kg −1 per day was supplied to DG treated mice for 8 weeks. AA intake at 10 or 20 mg kg −1 per day increased its deposit in brain. DG treatment increased Bax, cleaved caspase-3 protein expression and decreased Bcl-2 expression. AA intake at 10 and 20 mg kg −1 per day declined Bax, cleaved caspase-3 expression, and retained Bcl-2 expression. DG treatment decreased brain glutathione content and glutathione peroxidase activity; increased brain reactive oxygen species and protein carbonyl levels, and enhanced NAPDH oxidase expression. AA intake at test doses reversed these changes. DG treatment up-regulated the expression of advanced glycation end product (AGE), carboxymethyllysine, receptor of AGE (RAGE), mitogen-activated protein kinases and CD11b as well as increasing the interleukin (IL)-6 and tumor necrosis factor (TNF)-alpha release in the brain. AA intake at 5, 10 and 20 mg kg −1 per day lowered AGE and carboxymethyllysine expression, and at 10 and 20 mg kg −1 per day reduced RAGE production. AA intake dose-dependently suppressed p-p38 expression and lowered IL-6 and TNF-alpha levels, and at 10 and 20 mg kg −1 per day down-regulated p-JNK and CD11b expression. DG treatment declined brain-derived neurotropic factor (BDNF) expression and raised glial fibrillary acidic protein (GFAP) expression. AA intake at 20 mg kg −1 per day retained BDNF expression and at 10 and 20 mg kg −1 per day reduced GFAP expression. These findings indicated that the supplement of asiatic acid might be beneficial to the prevention or alleviation of brain aging.