Maternal exposure to a Western‐style diet causes differences in intestinal microbiota composition and gene expression of suckling mouse pups. Issue 1 (12th July 2016)

• Record Type: Journal Article
• Authors: Steegenga, Wilma T.; Mischke, Mona; Lute, Carolien; Boekschoten, Mark V.; Lendvai, Agnes; Pruis, Maurien G. M.; Verkade, Henkjan J.; van de Heijning, Bert J. M.; Boekhorst, Jos; Timmerman, Harro M.; Plösch, Torsten; Müller, Michael; Hooiveld, Guido J. E. J.
• Other Names: Espin Juan Carlos guestEditor.
• Is Part Of: Molecular nutrition & food research. Volume 61:Issue 1(2017)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2016-07-12
• Subjects: Gut development -- Maternal diet -- Microbiota composition -- Offspring -- Transcriptomics; Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.201600141
• Languages: English
• ISSNs: 1613-4125

Abstract

Abstract : Although the importance of nutritional programming during the first 1000 days is commonly acknowledged, the effect of the maternal diet on the developing gut is largely unknown. In this study we show that exposure of C57Bl/6 mouse dams to a Western‐style diet alters the microbiota composition in the colon of their two‐week‐old offspring.Furthermore, intestinal gene expression was affected by the maternal diet in a sexual dimorphic way. Correlation analysis revealed that these two effects occurred largely independently. Abstract : Scope: The long‐lasting consequences of nutritional programming during the early phase of life have become increasingly evident. The effects of maternal nutrition on the developing intestine are still underexplored. Methods and results: In this study, we observed (1) altered microbiota composition of the colonic luminal content, and (2) differential gene expression in the intestinal wall in 2‐week‐old mouse pups born from dams exposed to a Western‐style (WS) diet during the perinatal period. A sexually dimorphic effect was found for the differentially expressed genes in the offspring of WS diet‐exposed dams but no differences between male and female pups were found for the microbiota composition. Integrative analysis of the microbiota and gene expression data revealed that the maternal WS diet independently affected gene expression and microbiota composition. However, the abundance of bacterial families not affected by the WS diet ( Bacteroidaceae, Porphyromonadaceae, and Lachnospiraceae ) correlated with the expression of genes playing a key role in intestinal development and functioning (e.g. Pitx2 and Ace2 ). Conclusion: Our data reveal that maternal consumption of a WS diet during the perinatal period alters both gene expression and microbiota composition in the intestinal tract of 2‐week‐old offspring.